Research Paper Volume 15, Issue 16 pp 8367—8383
The novel angiogenesis regulator circFAM169A promotes the metastasis of colorectal cancer through the angiopoietin-2 signaling axis
Figure 5. circFAM169A promotes CRC by sponging miR-518a-5p. (A) Venn diagram of miRNA response elements in circBank, CircInteractome, and TargetScan that may interact with circFAM169A. (B) The luciferase activities of different plasmids were measured by a dual-luciferase assay. (C) RNA antisense purification (RAP) assay showed that miR-518a-5p was significantly enriched when circFAM169A was used as a probe. (D) Construction of a luciferase plasmid encoding mut-circFAM169A. (E) The luciferase activities of different groups were measured by a dual-luciferase assay. (F, G) Transwell migration assay and Transwell invasion assay were performed using HCT116 and RKO cells. (H, I) An in vitro Matrigel tube formation assay was performed to evaluate the angiogenic ability of HUVECs. The representative micrographs are shown at 200× magnification.