Research Paper Volume 15, Issue 17 pp 9086—9104
Integrated clustering signature of genomic heterogeneity, stemness and tumor microenvironment predicts glioma prognosis and immunotherapy response
Figure 10. SH2D4A affects proliferation and migration of glioma cells. (A) Immunohistochemical staining of SH2D4A in glioma (data from HPA). (B, C) qRT-PCR and western blot analysis of SH2D4A knockdown efficiency in U87 MG cells. (D) Analysis of proliferation of control and SH2D4A-deficient U87 MG cells by CCK8 assay. (E, F) Representative images and statistical analysis of EdU assay in control and SH2D4A-deficient U87 MG cells. (G, H) Representative images and statistical analysis of cell migration assay in control and SH2D4A-deficient U87 MG cells at the indicated times. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.