Research Paper Volume 15, Issue 19 pp 10681—10704
Analysis of potential biomarkers for diabetic kidney disease based on single-cell RNA-sequencing integrated with a single-cell sequencing assay for transposase-accessible chromatin
Figure 4. Single-cell chromatin accessibility analysis of db/db and db/m mice. (A) tSNE of scATAC-seq data from db/db and db/m mice kidney cells, colored by cell type. B cell, B lymphocytes; CD, collecting duct; DCT, distal convoluted tubule; Endo, endothelial cells; LOH, loop of Henle; M1, classical macrophage; M2, alternatively activated macrophage; NK, natural killer cell; Neut, neutrophil progenitor; Podo, podocyte; PTB, proximal tubule brush; PTS1, first segment of the proximal tubule; PTS2, second segment of the proximal tubule; PTS3, third segment of the proximal tubule; T cell, T lymphocytes. (B) Cell type-specific peaks in scATAC-seq data. (C) Cell type-specific transcription factor (TF) expression based on integrative analysis of scATAC-seq and scRNA-seq data. (D) Browser tracks of marker genes from scRNA-seq data in each cell type based on scATAC-seq data. (E) Heatmap showing the expression of cell type-specific positive TFs in each cluster, with the expression level indicated by color intensity.