Research Paper Volume 15, Issue 21 pp 11860—11874
Lipid reprogramming induced by the NNMT-ABCA1 axis enhanced membrane fluidity to promote endometrial cancer progression
Figure 5. Cholesterol is lowered thanks to ABCA1 expression being boosted by NNMT, triggering EMT in turn. EMT-related protein western blotting result (A) as an example. Quantitative real-time polymerase chain reaction analysis of relative mRNA levels relating to the epigenetic regulatory transition (EMT) (B–D). (E) Western blotting and quantitative analysis of relative EMT-related protein levels in DoTc2 cells transfected with anti-ABCA1 siRNA; EMT-related protein levels are shown as a representative result. All experiments were conducted three times. The data are presented as a mean, standard error of the mean. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).