Research Paper Volume 15, Issue 21 pp 12537—12550

Figure 3. Colocalization of HuR and Iba-1 in mice hippocampi with immunofluorescence. (A) The colocalization of HuR and Iba-1 in mice hippocampi with immunofluorescence. (B) The amount of the HuR protein expression positive rate in the CA1 region of the hippocampus, as determined using immunofluorescence staining. (C) The intensity of Iba-1 in the CA1 region of the hippocampus, as determined using immunofluorescence staining. (D) The levels of TNF-α in five groups of mice hippocampi, as measured using ELISA. (E) The levels of IL-1β in five groups of mice hippocampi, as measured using ELISA. The data present the means ± standard error of the mean. (n=3 per group). #p <0.05 versus the control group; *p<0.05 versus the MTX group. Representative micrographs: ×400 magnification.