Research Paper Volume 15, Issue 23 pp 13693—13709
The antioxidant Glycitin protects against intervertebral disc degeneration through antagonizing inflammation and oxidative stress in nucleus pulposus cells
Figure 4. Glycitin alleviated TNF-α-induced oxidative stress and mitochondrial dysfunction in HNPCs. (A, B) JC-1 assay was applied to detect the mitochondrial membrane potential of human NP cells in every group (n=3). Scale bar: 100μm. (C, D) ROS levels of human NP cells were detected with DCFDA (n=3). Scale bar: 100 μm. The values shown represent the mean ± SD of three independent experiments. *p < 0.05 vs. control group.