Research Paper Volume 15, Issue 24 pp 14845—14863

A comprehensive analysis of the prognostic and immunotherapeutic characteristics of KIFC1 in pan-cancer and its role in the malignant phenotype of pancreatic cancer

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Figure 8. Effects of KIFC1 on malignant phenotypes of PANC-1 and SW1990 cells. (A) Immunohistochemistry of KIFC1 in pancreatic cancer tumor tissues and corresponding adjacent normal tissues. (B) Western blot analysis of KIFC1 knockdown. (C) Transwell for testing migration and invasion ability. (D) Scratch assay for determining cell migration. (E, F) Proliferation attenuation of PANC-1 and SW1990 cells was detected by colony formation assay. (G, H) Proliferation attenuation of PANC-1 and SW1990 cells was detected by Edu assay. Ns: p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001. ****p < 0.0001.