Research Paper Volume 15, Issue 24 pp 14915—14929
hsa_circ_0002980 prevents proliferation, migration, invasion, and epithelial-mesenchymal transition of liver cancer cells through microRNA-1303/cell adhesion molecule 2 axis
Figure 5. circ_0002980 overexpression dramatically prevented the migration, invasion, and EMT of LC cells through miR-1303. miR-1303 mimics and circ_0002980 overexpression plasmids were transfected into HepG2 and Huh7 cells, respectively. (A) After transfection, Transwell assay was performed to certify the change in cell migration ability. Magnification, 200×. (B) The invasion capacity of the processed cells was observed by Transwell assay. Magnification, 200×. (C) The levels of EMT-related proteins in the transfected HepG2 and Huh7 cells were monitored using Western blot. (D) The expression levels of four proteins were calculated in line with the grey values. **P < 0.01; ***P < 0.001.