Research Paper Volume 15, Issue 24 pp 15324—15339

Figure 3. Different motoneurons in the spinal cord of ALS mice with different onset time. Different MNs are stained with different markers and colors, FαMNs (MMP-9⁺), SαMNs (ChAT⁺NeuN⁺MMP-9⁻) and (ChAT⁺NeuN⁻MMP-9⁻), with MMP-9⁺ (red), ChAT⁺ (green) and NeuN⁺ (blue). Scale bar = 100 μM (A) and the quantitative results (B). The yellow boxes represent the typical positive cells (enlarged approximate twice on the original position). The typical MMP-9⁺, ChAT⁺NeuN⁺MMP-9⁻ and ChAT⁺NeuN⁻MMP-9⁻ positive cells are enlarged approximately 4 times and shown on the bottom right panel. ^&&&P < 0.001 vs. control at 50~60 days. ^***P < 0.001 vs. control at 90~100 days. ^###P < 0.001 vs. control at 130~140 days. In all experiments, half male and half female mice were used in each group (n = 6).