Research Paper Volume 16, Issue 2 pp 1938—1951
Long noncoding RNA MEG3 regulates cell proliferation and apoptosis by disrupting microRNA-9-5p-mediated inhibition of NDRG1 in prostate cancer
Figure 6. LncMEG3 inhibits proliferation and promotes apoptosis in PCa cells by relieving the suppression effects of miR-9-5p on NDRG1. (A) Colony forming assay performed in PC3 cells to evaluate cell proliferation ability. (B, C) Cell viability of PC3 cells after overexpressing lncMEG3 and miR-9-5p or knocking down NDRG1 were determined using CCK8 assays. EdU assays also implicated the same results. (D, E) Cell cycle distribution and apoptosis were analysed by flow cytometry in PC3 cells. (F) Western blot analysis showed alterations in related proteins, * P < 0.05.