Research Paper Volume 16, Issue 6 pp 5091—5107

Figure 3. The exploration of downstream mechanism of GSG2-regulating thyroid cancer. (A) The mRNA levels of top 13 co-expressed genes of GSG2 were detected through qRT-PCR experiment. (B) The protein levels of BIRC5, AURKB and CDCA8 were analyzed in response to GSG2 downregulation by western blot. (C) Co-IP assay demonstrated an endogenous interaction between GSG2 and SMURF1. (D) The E3 ubiquitin ligases of AURKB. (E) The half-life of AURKB protein in GSG2-depleted B-CPAP and TPC-1 cells was assessed at 2 h, 4 h and 8h following 0.2 mg/mL CHX treatment. (F) After MG-132 addition, AURKB protein levels in GSG2-depleted B-CPAP and TPC-1 cells were measured. (G) The ubiquitination level of AURKB was detected in GSG2-depleted B-CPAP cells after immunoprecipitation using AURKB or IgG antibodies.